Optimizing RT-PCR with Suitable Primers

Which DNA oligonucleotide pair could serve as a suitable primer set in an RT-PCR amplification of this transcript?

The oligonucleotides are written in the 5' to 3' direction.

Primer set 2

(Primer 1: AGTCAAGGTCTCC, Primer 2: GTTACGTGGAGCG) could serve as a suitable primer set in an RT-PCR amplification of the transcript.

The selection of suitable primers for RT-PCR is crucial for the specific amplification of the target transcript. In Primer set 2, Primer 1 (AGTCAAGGTCTCC) and Primer 2 (GTTACGTGGAGCG) are designed to anneal to complementary sequences on the target RNA. The forward primer (Primer 1) binds to the RNA template at the 3' end and the reverse primer (Primer 2) at the 5' end, ensuring that the amplified cDNA spans the entire transcript.

The selected primers have appropriate length and GC content, and their sequences are specific to the target transcript, minimizing non-specific amplification. Additionally, the primers are written in the 5' to 3' direction, reflecting the direction of DNA synthesis during the polymerase chain reaction (PCR).

Overall, Primer set 2 is designed to provide specificity and efficiency in the amplification of the target transcript in the context of reverse transcription PCR (RT-PCR).